Anti-SIRP alpha Antibody [15-414] (A279025)

$340

Mouse monoclonal [15-414] antibody to SIRP alpha for Flow Cytometry and IHC-Fr.

Alternative Formats and ConjugatesAlternative Formats

PE
Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-SIRP alpha Antibody [15-414]
Description
Mouse monoclonal [15-414] antibody to SIRP alpha.
Specificity
This antibody recognises human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells.CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
Applications
Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:100 for 0.1 mg, 1:25 for 0.2 mg - 1:200 for 0.1 mg, 1:100 for 0.2 mg, IHC-Fr: 1:100, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human
Immunogen
Monocyte derived dendritic cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
15-414
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD172a antibody, clone 15-414 recognizes human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells.CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
Synonyms
Bit, Brain Ig like molecule with tyrosine based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, Brain immunoglobulin like molecule with tyrosine based activation motifs, CD172 antigen like family member A, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MYD 1, Myd 1 antigen, MyD-1 antigen, p84, Protein tyrosine phosphatase non receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SHPS1_HUMAN, Signal regulatory protein alpha, Signal regulatory protein alpha 2, Signal regulatory protein alpha 3, Signal regulatory protein alpha type 1, Signal regulatory protein alpha type 2, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRP, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, SIRPA, SIRPalpha, SIRPalpha1, SIRPalpha2, SIRPalpha3, Tyrosine phosphatase SHP substrate 1, Tyrosine protein phosphatase non receptor type substrate 1, Tyrosine-protein phosphatase non-receptor type substrate 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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