Anti-SIRP alpha Antibody [15-414] (A279025)

Anti-SIRP alpha Antibody [15-414]

Mouse monoclonal [15-414] antibody to SIRP alpha.

This antibody recognises human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells.CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:100 for 0.1 mg, 1:25 for 0.2 mg - 1:200 for 0.1 mg, 1:100 for 0.2 mg, IHC-Fr: 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Human

Monocyte derived dendritic cells.

Mouse

Monoclonal

15-414

IgG2a

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD172a antibody, clone 15-414 recognizes human CD172a, also known as signal-regulatory protein alpha, a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells.CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.

Bit, Brain Ig like molecule with tyrosine based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, Brain immunoglobulin like molecule with tyrosine based activation motifs, CD172 antigen like family member A, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MYD 1, Myd 1 antigen, MyD-1 antigen, p84, Protein tyrosine phosphatase non receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SHPS1_HUMAN, Signal regulatory protein alpha, Signal regulatory protein alpha 2, Signal regulatory protein alpha 3, Signal regulatory protein alpha type 1, Signal regulatory protein alpha type 2, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRP, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, SIRPA, SIRPalpha, SIRPalpha1, SIRPalpha2, SIRPalpha3, Tyrosine phosphatase SHP substrate 1, Tyrosine protein phosphatase non receptor type substrate 1, Tyrosine-protein phosphatase non-receptor type substrate 1