Anti-Siglec 7 Antibody [5-386] (A279400)

Anti-Siglec 7 Antibody [5-386]

Mouse monoclonal [5-386] antibody to Siglec 7.

This antibody recognises human CDw328, a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-7, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. CDw328 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:100 - 1:200, IHC-Fr: 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Human

Monocyte derived dendritic cells.

Mouse

Monoclonal

5-386

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CDw328 antibody, clone 5-386 recognizes human CDw328, a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-7, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase. CDw328 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.

Adhesion inhibitory receptor molecule 1, Adhesion inhibitory receptor molecule 1, siglec 7, AIRM 1, AIRM-1, AIRM1, CD328, CD328 antigen, CDw328, D siglec, D-siglec, HLDA 8, p75, p75/AIRM1, QA79, QA79 membrane protein, Sialic acid binding Ig like lectin 7, Sialic acid binding immunoglobulin like lectin 7, Sialic acid-binding Ig-like lectin 7, SIGL7_HUMAN, Siglec-7, SIGLEC19P, SIGLEC7, SIGLECP2