Anti-SIAH1 Antibody (A14007)

Name: Anti-SIAH1 Antibody
Brand: Antibodies.com
SKU: A14007
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SIAH1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SIAH1 Antibody

Description

Rabbit polyclonal antibody to SIAH1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 140-282 of human SIAH1 (NP_003022.3).

Sequence

SLDAVMPHLMHQHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGINVTISMC

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

31 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 ubiquitin-protein ligase SIAH1, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, Seven in absentia homolog 1 (Drosophila), Siah 1, Siah 1a, Siah E3 ubiquitin protein ligase 1, Siah-1, Siah-1a, SIAH1A, SIAH1_HUMAN, Ubiquitin ligase SIAH1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SIAH1 Antibody (A14007)

Western blot analysis of extracts of mouse kidney, using Anti-SIAH1 Antibody (A14007) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Figure 2: Immunohistochemistry - Anti-SIAH1 Antibody (A14007)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-SIAH1 Antibody (A14007) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SIAH1 Antibody (A14007)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SIAH1 Antibody (A14007) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SIAH1 Antibody (A14007)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-SIAH1 Antibody (A14007) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.