Rabbit polyclonal antibody to SHP-1 (Phospho-Tyr564)
Specificity
The antibody detects endogenous levels of SHP-1 only when phosphorylated at tyrosine 564.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 564(D-V-Y(p)-E-N) derived from Human SHP-1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
NR0B2, Nr0b2a, NR0B2_HUMAN, Nuclear receptor subfamily 0 group B member 2, Nuclear receptor subfamily 0, group B, member 2a, Orphan nuclear receptor SHP, SHP, Shp1, Small heterodimer partner
Western blot analysis of extracts from COS7 cells treated with EGF using SHP-1 (Phospho-Tyr564) Antibody #.The lane on the right is treated with the antigen-specific peptide.
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