Anti-SHIP-1 Antibody (A8360)

Name: Anti-SHIP-1 Antibody
Brand: Antibodies.com
SKU: A8360
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SHIP-1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SHIP-1 Antibody

Description

Rabbit polyclonal antibody to SHIP-1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human SHIP1 (NP_001017915.1).

Sequence

PPCSGSSITEIINPNYMGVGPFGPPMPLHVKQTLSPDQQPTAWSYDQPPKDSPLGPCRGESPPTPPGQPPISPKKFLPSTANRGLPPRTQESRPSDLGKNA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

4, 5-trisphosphate 5-phosphatase 1, hp51CN, Inositol polyphosphate 5 phosphatase, Inositol polyphosphate 5 phosphatase 145kDa, Inositol polyphosphate 5 phosphatase of 145kDa, Inositol polyphosphate-5-phosphatase of 145 kDa, INPP5D, MGC104855, MGC142140, MGC142142, p150Ship, Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1, Phosphatidylinositol-3, SH2 containing inositol phosphatase isoform b, SH2 domain containing inositol 5' phosphatase 1, SH2 domain containing inositol phosphatase 1, SH2 domain-containing inositol phosphatase 1, SH2 domain-containing inositol-5''-phosphatase 1, SHIP, SHIP1, SHIP1_HUMAN, Signaling inositol polyphosphate 5 phosphatase SIP 145, SIP-145, SIP145

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SHIP-1 Antibody (A8360)

Western blot analysis of extracts of Mouse spleen, using Anti-SHIP-1 Antibody (A8360) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-SHIP-1 Antibody (A8360)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-SHIP-1 Antibody (A8360) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-SHIP-1 Antibody (A8360)

Immunofluorescence analysis of mouse spleen cells using Anti-SHIP-1 Antibody (A8360) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-SHIP-1 Antibody (A8360)

Immunofluorescence analysis of THP-1 cells using Anti-SHIP-1 Antibody (A8360) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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