Anti-Shc1 (Phospho-Tyr427) Antibody (A50200)

Name: Anti-Shc1 (Phospho-Tyr427) Antibody
Brand: Antibodies.com
SKU: A50200
Price: 265.0 USD
Availability: InStock

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$265

Rabbit polyclonal antibody to Shc1 (Phospho-Tyr427) for WB, IHC and IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Shc1 (Phospho-Tyr427) Antibody

Description

Rabbit polyclonal antibody to Shc1 (Phospho-Tyr427)

Specificity

The antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 427.

Applications

WB, IHC, IF

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1.

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1.0mg / ml

Formulation

Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Synonyms

FLJ26504, p66, p66SHC, SH2 domain protein C1, SHC, SHC (Src homology 2 domain containing) transforming protein 1, SHC 1, SHC A, SHC adaptor protein 1, SHC transforming protein, SHC transforming protein 1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHC1_HUMAN, SHCA, Src homology 2 domain-containing-transforming protein C1

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Anti-Shc1 (Phospho-Tyr427) Antibody (A50200)

Western blot analysis of extracts from 293 cells untreated or treated with PMA using Shc1 (Phospho-Tyr427) Antibody #11317.

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Figure 2: Immunohistochemistry - Anti-Shc1 (Phospho-Tyr427) Antibody (A50200)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1 (Phospho-Tyr427) Antibody #11317 (left) or the same antibody preincubated with blocking peptide (right).