Anti-Shc (phospho Tyr427) Antibody (A94644)

Name: Anti-Shc (phospho Tyr427) Antibody
Brand: Antibodies.com
SKU: A94644
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to Shc (phospho Tyr427) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Shc (phospho Tyr427) Antibody

Description

Rabbit polyclonal antibody to Shc (phospho Tyr427).

Specificity

This antibody detects endogenous levels of Shc only when phosphorylated at Tyr427.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Shc around the phosphorylation site of Tyr427 (amino acids 393-442).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

62kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

FLJ26504, p66, p66SHC, SH2 domain protein C1, SHC (Src homology 2 domain containing) transforming protein 1, SHC 1, SHC A, SHC adaptor protein 1, SHC transforming protein, SHC transforming protein 1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHC1, SHC1_HUMAN, SHCA, Src homology 2 domain-containing-transforming protein C1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Shc (phospho Tyr427) Antibody (A94644)

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5' using Anti-Shc (phospho Tyr427) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-Shc (phospho Tyr427) Antibody (A94644)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-Shc (phospho Tyr427) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Western Blot - Anti-Shc (phospho Tyr427) Antibody (A94644)

Western blot analysis of various cells using Anti-Shc (phospho Tyr427) Antibody.

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Figure 4: Immunohistochemistry - Anti-Shc (phospho Tyr427) Antibody (A94644)

Immunohistochemical analysis of paraffin-embedded human colon cancer using Anti-Shc (phospho Tyr427) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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