Rabbit polyclonal antibody to SHC (phospho Tyr427).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around Y427 of human SHC1 (NP_892113.4).
Sequence
PSYVN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
50 kDa / 55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
SH2 domain protein C1, SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHC1, SHCA, Src homology 2 domain-containing-transforming protein C1
Western Blot - Anti-SHC (phospho Tyr427) Antibody (A308256)
Western blot analysis of extracts of A-431 cells, using Anti-SHC (phospho Tyr427) Antibody (A308256) at 1:1,000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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