Anti-SHANK3 Antibody (A307131)

Name: Anti-SHANK3 Antibody
Brand: Antibodies.com
SKU: A307131
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SHANK3 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SHANK3 Antibody

Description

Rabbit polyclonal antibody to SHANK3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SHANK3 (NP_277052.1).

Sequence

MDGPGASAVVVRVGIPDLQQTKCLRLDPAAPVWAAKQRVLCALNHSLQDALNYGLFQPPSRGRAGKFLDEERLLQEYPPNLDTPLPYLEFRYKRRVYAQNLIDDKQFAKLHTKANLKKFMDYVQLHSTDKVARLLDKGLDPNFHDPDSGE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

220 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AI841104, DEL22q13.3, KIAA1650, Proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2, SH3 and multiple ankyrin repeat domains 3, SH3 and multiple ankyrin repeat domains protein 3, SH3/ankyrin domain gene 3, SHAN3_HUMAN, Shank postsynaptic density protein, Shank3b, SPANK 2, SPANK2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SHANK3 Antibody (A307131)

Western blot analysis of extracts of Mouse brain, using Anti-SHANK3 Antibody (A307131) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-SHANK3 Antibody (A307131)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-SHANK3 Antibody (A307131) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SHANK3 Antibody (A307131)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-SHANK3 Antibody (A307131) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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