Bif-1 expression in HEK293 (A) and A431 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Bif-1 Antibody (A83782) at 0.1µg/ml and detected by chemiluminescence.
Bif-1 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Bif-1 Antibody (A83782) at 0.1µg/ml and detected by chemiluminescence.
Bif-1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Bif-1 Antibody (A83782) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
Bif-1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Bif-1 Antibody (A83782) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
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