Anti-SGK (phospho Ser422) Antibody (A94646)

Name: Anti-SGK (phospho Ser422) Antibody
Brand: Antibodies.com
SKU: A94646
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to SGK (phospho Ser422) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-SGK (phospho Ser422) Antibody

Description

Rabbit polyclonal antibody to SGK (phospho Ser422).

Specificity

This antibody detects endogenous levels of SGK only when phosphorylated at Ser422.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:40000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human SGK around the phosphorylation site of Ser422 (amino acids 381-430).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

48kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

OTTHUMP00000017247, Serine/threonine protein kinase SGK, Serine/threonine protein kinase Sgk1, Serine/threonine-protein kinase Sgk1, Serum and glucocorticoid regulated kinase, Serum/glucocorticoid regulated kinase, Serum/glucocorticoid regulated kinase 1, Serum/glucocorticoid-regulated kinase 1, SGK, SGK 1, Sgk1 variant i3, SGK1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SGK (phospho Ser422) Antibody (A94646)

Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15' using Anti-SGK (phospho Ser422) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-SGK (phospho Ser422) Antibody (A94646)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-SGK (phospho Ser422) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Western Blot - Anti-SGK (phospho Ser422) Antibody (A94646)

Western blot analysis of VEC HY926 cells using Anti-SGK (phospho Ser422) Antibody.

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Figure 4: Immunohistochemistry - Anti-SGK (phospho Ser422) Antibody (A94646)

Immunohistochemical analysis of paraffin-embedded human breast cancer using Anti-SGK (phospho Ser422) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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