Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Frizzled protein, Frizzled protein human endometrium, FRP 4, FRP4, FrpHE, human endometrium, PYL, Secreted frizzled-related protein 4, sFRP-4, SFRP4_HUMAN
Figure 1: Western Blot - Anti-SFRP4 Antibody [ARC0923] (A305346)
Western blot analysis of extracts of various cell lines, using Anti-SFRP4 Antibody [ARC0923] (A305346) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-SFRP4 Antibody [ARC0923] (A305346) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Anti-SFRP4 Antibody [ARC0923] (A305346) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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