Anti-SF3B3 Antibody [ARC1667] (A309080)

Name: Anti-SF3B3 Antibody [ARC1667]
Brand: Antibodies.com
SKU: A309080
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1667] antibody to SF3B3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-SF3B3 Antibody [ARC1667]

Description

Rabbit monoclonal [ARC1667] antibody to SF3B3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1101-1217 of human SF3B3/SAP130 (Q15393).

Sequence

VLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEVSKKLEDIRTRYAF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1667

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

136 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

KIAA0017, Pre mRNA splicing factor SF3b 130 kDa subunit, Pre-mRNA-splicing factor SF3b 130 kDa subunit, RSE1, SAP 130, SAP130, SF3b130, SF3B3_HUMAN, Spliceosome associated protein 130, Spliceosome-associated protein 130, Splicing factor 3B subunit 3, Splicing factor 3b subunit 3 130kD, STAF130

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SF3B3 Antibody [ARC1667] (A309080)

Western blot analysis of extracts of various cell lines, using Anti-SF3B3 Antibody [ARC1667] (A309080) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-SF3B3 Antibody [ARC1667] (A309080)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-SF3B3 Antibody [ARC1667] (A309080) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SF3B3 Antibody [ARC1667] (A309080)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-SF3B3 Antibody [ARC1667] (A309080) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SF3B3 Antibody [ARC1667] (A309080)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SF3B3 Antibody [ARC1667] (A309080) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.