Anti-Serum Response Factor SRF Antibody (A15859)

Name: Anti-Serum Response Factor SRF Antibody
Brand: Antibodies.com
SKU: A15859
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Serum Response Factor SRF for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Serum Response Factor SRF Antibody

Description

Rabbit polyclonal antibody to Serum Response Factor SRF.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S103 of human SRF (NP_003122.1).

Sequence

SLSEM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

73 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

c fos serum response element binding factor, c fos serum response element binding transcription factor, ELK3, ERP, MCM 1, MCM1, OTTHUMP00000039820, SAP2, Serum response factor, SRF, SRF serum response factor c fos serum response element binding transcription factor, SRF_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Serum Response Factor SRF Antibody (A15859)

Western blot analysis of A-431, using Anti-Serum Response Factor SRF Antibody (A15859) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-Serum Response Factor SRF Antibody (A15859)

Western blot analysis of various lysates, using Anti-Serum Response Factor SRF Antibody (A15859) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-Serum Response Factor SRF Antibody (A15859)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Serum Response Factor SRF Antibody (A15859) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Serum Response Factor SRF Antibody (A15859)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-Serum Response Factor SRF Antibody (A15859) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.