Anti-SERCA2 Antibody (A82919)

Name: Anti-SERCA2 Antibody
Brand: Antibodies.com
SKU: A82919
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to SERCA2 for ELISA, WB, IF and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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Tags:

ER Marker Nuclear Marker Plasma Membrane Marker

Specifications

Name

Anti-SERCA2 Antibody

Description

Goat polyclonal antibody to SERCA2.

Applications

ELISA, WB, IF, IHC

Dilutions

ELISA: 1:128,000, WB: 0.1-0.3µg/ml, IHC: 5µg/ml, IF: 10µg/ml

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the internal region of human SERCA2.

Sequence

DELNPSAQRDACLN

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

110 kDa

Predicted MW

110kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

AT2A2_HUMAN, Atp2a2, ATP2B, ATPase Ca++ transporting cardiac muscle slow twitch 2, Calcium pump 2, Calcium-transporting ATPase sarcoplasmic reticulum type, Calcium-transporting ATPase sarcoplasmic reticulum type slow twitch skeletal muscle isoform, Cardiac Ca2+ ATPase, DAR, DD, Endoplasmic reticulum class 1/2 Ca(2+) ATPase, MGC45367, Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, SERCA 2, SERCA2 ATPase, serca2a, slow twitch skeletal muscle isoform, SR Ca(2+)-ATPase 2

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SERCA2 Antibody (A82919)

SERCA2 expression in Human (A), Mouse (B) and Rat (C) Heart lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SERCA2 Antibody (A82919) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-SERCA2 Antibody (A82919)

SERCA2 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SERCA2 Antibody (A82919) at 5µg/ml and revealed with alkaline phosphatase (AP).

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Figure 3: Immunofluorescence - Anti-SERCA2 Antibody (A82919)

SERCA2 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-SERCA2 Antibody (A82919) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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