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Anti-Semaphorin 3c Antibody (A91344)

Western Blot - Anti-Semaphorin 3c Antibody (A91344) - Antibodies.com Immunofluorescence - Anti-Semaphorin 3c Antibody (A91344) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Semaphorin 3c for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Semaphorin 3c Antibody
Description
Rabbit polyclonal antibody to Semaphorin 3c.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 21-330 of human SEMA3C (NP_006370.1).
Sequence
GSSQPQARVYLTFDELRETKTSEYFSLSHHPLDYRILLMDEDQDRIYVGSKDHILSLNINNISQEALSVFWPASTIKVEECKMAGKDPTHGCGNFVRVIQTFNRTHLYVCGSGAFSPVCTYLNRGRRSEDQVFMIDSKCESGKGRCSFNPNVNTVSVMINEELFSGMYIDFMGTDAAIFRSLTKRNAVRTDQHNSKWLSEPMFVDAHVIPDGTDPNDAKVYFFFKEKLTDNNRSTKQIHSMIARICPNDTGGLRSLVNKWTTFLKARLVCSVTDEDGPETHFDELEDVFLLETDNPRTTLVYGIFTTSSS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
85 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Sema E, SEMA3C, semaE, Semahorin3C, Semaphorin E, SemaphorinE
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Semaphorin 3c Antibody (A91344) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Semaphorin 3c Antibody (A91344)
Western blot analysis of Human serum, using Anti-Semaphorin 3c Antibody (A91344) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-Semaphorin 3c Antibody (A91344) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Semaphorin 3c Antibody (A91344)
Immunofluorescence analysis of L929 cells using Anti-Semaphorin 3c Antibody (A91344) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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