Anti-SEMA4F Antibody (A12936)

Name: Anti-SEMA4F Antibody
Brand: Antibodies.com
SKU: A12936
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SEMA4F for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SEMA4F Antibody

Description

Rabbit polyclonal antibody to SEMA4F.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 280-420 of human SEMA4F (NP_004254.2).

Sequence

RKTLQQRWTTFLKADLLCPGPEHGRASSVLQDVAVLRPELGAGTPIFYGIFSSQWEGATISAVCAFRPQDIRTVLNGPFRELKHDCNRGLPVVDNDVPQPRPGECITNNMKLRHFGSSLSLPDRVLTFIRDHPLMDRPVFP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

83 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

M SEMA, m Sema M, PRO2353, SEM4F_HUMAN, Sema domain immunoglobulin domain (Ig) transmembrane, Sema M, Sema W, SEMAM, Semaphorin M, Semaphorin W, Semaphorin-4F, Semaphorin-M, Semaphorin-W, SEMAW

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-SEMA4F Antibody (A12936)

Western blot analysis of extracts of various cell lines, using Anti-SEMA4F Antibody (A12936) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-SEMA4F Antibody (A12936)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-SEMA4F Antibody (A12936) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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