Immunofluorescent analysis of a rat brain cerebellum section stained with Anti-Secretagogin Antibody (A85357), dilution 1:1,000, in green, and co-stained with Anti-Calbindin Antibody (A85360), dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. Anti-Secretagogin Antibody stains granule cells and interneurons while calbindin antibody reveals proteins expressed in dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum.
Figure 2: Western Blot - Anti-Secretagogin Antibody (A85357)
Western blot analysis of different tissue lysates using Anti-Secretagogin Antibody (A85357), dilution 1:1,000, in green. The lanes contain: [1] protein standard (red), [2] rat pancreas, [3] rat olfactory bulb, and [4] mouse olfactory bulb lysates. Bands at ~27 kDa correspond to secretagogin protein.
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