Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Protein translocation complex beta, Protein transport protein SEC61 beta subunit, Protein transport protein Sec61 subunit beta, SC61B_HUMAN, SEC 61B, SEC61 B, Sec61 beta subunit, Sec61 complex beta subunit
Figure 1: Western Blot - Anti-SEC61B Antibody (A307609)
Western blot analysis of extracts of various cell lines, using Anti-SEC61B Antibody (A307609) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunofluorescence analysis of NIH/3T3 cells using Anti-SEC61B Antibody (A307609) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of PC-12 cells using Anti-SEC61B Antibody (A307609) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of U2OS cells using Anti-SEC61B Antibody (A307609) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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