Anti-SEC61A Antibody (A80623)

Name: Anti-SEC61A Antibody
Brand: Antibodies.com
SKU: A80623
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SEC61A for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Ribosomal Marker

Specifications

Name

Anti-SEC61A Antibody

Description

Rabbit polyclonal antibody to SEC61A.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 310-420 of human SEC61A1 (NP_037468.1).

Sequence

ARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCYYLSPPESFGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

chunp6898, fb62c11, HSEC61, protein transport protein SEC61 alpha subunit, protein transport protein Sec61 subunit alpha, Protein transport protein Sec61 subunit alpha isoform 1, S61A1_HUMAN, SEC 61, SEC 61A, SEC61, Sec61 alpha 1, Sec61 alpha 1 subunit, Sec61 alpha 1 subunit (S. cerevisiae), Sec61 alpha-1, Sec61 homolog, SEC61A1, sec61aa

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SEC61A Antibody (A80623)

Western blot analysis of extracts of A-549 cells, using Anti-SEC61A Antibody (A80623) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-SEC61A Antibody (A80623)

Immunofluorescence analysis of HeLa cells using Anti-SEC61A Antibody (A80623) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-SEC61A Antibody (A80623)

Immunofluorescence analysis of NIH/3T3 cells using Anti-SEC61A Antibody (A80623) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-SEC61A Antibody (A80623)

Immunofluorescence analysis of PC-12 cells using Anti-SEC61A Antibody (A80623) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).

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