Anti-SCRIBBLE Antibody (A121159)

Name: Anti-SCRIBBLE Antibody
Brand: Antibodies.com
SKU: A121159
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to SCRIBBLE for ELISA, WB and IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SCRIBBLE Antibody

Description

Goat polyclonal antibody to SCRIBBLE.

Applications

ELISA, WB, IF

Dilutions

ELISA: 1:1,000, WB: 1-3µg/ml, IF: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human SCRIBBLE.

Sequence

C-PEGPGKEKELPGQ

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

200-250 kDa

Predicted MW

178kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CRIB 1, CRIB1, hScrib, LAP 4, LAP4, PDZ domain protein scribble, Protein LAP4, Protein scribble homolog, SCRB 1, SCRB1, Scrib, SCRIB 1, SCRIB1, Scribble homolog 1, scribbled homolog (Drosophila), SCRIB_HUMAN, Vartul

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-SCRIBBLE Antibody (A121159)

Anti-AADACL1 Antibody (A121150) - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).

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Figure 2: Immunofluorescence - Anti-SCRIBBLE Antibody (A121159)

Anti-AADACL1 Antibody (A121150) - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).

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Figure 3: Flow Cytometry - Anti-SCRIBBLE Antibody (A121159)

Anti-AADACL1 Antibody (A121150) - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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