Western blot analysis of extracts of various cell lines, using SCG3 antibody (A7799) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST.