This antibody recognises human stem cell factor (SCF), a type I transmembrane protein and haematopoietic growth factor, which acts in synergy with other growth factors and signals through the c-Kit receptor.Interaction between SCF and the receptor tyrosine kinase c-Kit is an essential step for the proliferation, survival and differentiation of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. The association between SCF and c-Kit is also involved with the progression and invasiveness of tumours, and deficiencies of either SCF or c-Kit result in mast cell deficiency and macrocytic anaemia.

Applications

WB, IHC-P, ELISA, Functional Assays

Dilutions

ELISA: 0.5 - 2 µg/ml, Functional Assays: 0.008 - 0.012 µg/ml, WB: 0.1 - 0.2 µg/ml

Reactivity

Human

Immunogen

Recombinant human stem cell factor.

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Concentration

1 mg/ml after reconstitution.

Product Form

Lyophilized

Reconstitution

Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide.

Formulation

Supplied in Phosphate Buffered Saline.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Goat anti Human Stem Cell Factor antibody recognizes human stem cell factor (SCF), a type I transmembrane protein and haematopoietic growth factor, which acts in synergy with other growth factors and signals through the c-Kit receptor.Interaction between SCF and the receptor tyrosine kinase c-Kit is an essential step for the proliferation, survival and differentiation of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development. The association between SCF and c-Kit is also involved with the progression and invasiveness of tumours, and deficiencies of either SCF or c-Kit result in mast cell deficiency and macrocytic anaemia.

Synonyms

C kit ligand, C-kit ligand, Ckit ligand , DCUA, DFNA69, DKFZp686F2250, familial progressive hyperpigmentation 2, FPH2, FPHH, KIT ligand, Kitl, KITLG, KL 1, KL1, Mast cell growth factor, MGF, MGF stem cell factor, SCF_HUMAN, SF, SHEP7, sKITLG, Soluble KIT ligand, Steel factor, steel, mouse, homolog of, Stem cell factor, Stem cell factor precursor

Isotype Controls