Anti-SAV1 Antibody (A306668)

Name: Anti-SAV1 Antibody
Brand: Antibodies.com
SKU: A306668
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to SAV1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-SAV1 Antibody

Description

Rabbit polyclonal antibody to SAV1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 199-383 of human SAV1 (NP_068590.1).

Sequence

LPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1700040G09Rik, 45 kDa WW domain protein, hWW 45, hWW45, Protein salvador homolog 1, salvador family WW domain containing protein 1, Salvador homolog 1, salvador homolog 1 (Drosophila), Salvador, Drosophila, homolog of, SAV, SAV 1, SAV1_HUMAN, WW 45, WW domain containing, WW domain-containing adaptor 45, WW domain-containing protein, 45-KD, WW45, WW45 protein, WWP 4, WWP4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SAV1 Antibody (A306668)

Western blot analysis of extracts from normal (control) and [KO Validated] SAV1 knockout (KO) HeLa cells, using Anti-SAV1 Antibody (A306668) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-SAV1 Antibody (A306668)

Western blot analysis of extracts of 293T cells, using Anti-SAV1 Antibody (A306668) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-SAV1 Antibody (A306668)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-SAV1 Antibody (A306668) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SAV1 Antibody (A306668)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-SAV1 Antibody (A306668) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.