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Anti-SARS-CoV-2 Spike Protein Antibody [4B9] (A254398)

Western Blot - Anti-SARS-CoV-2 Spike Protein Antibody [4B9] (A254398) - Antibodies.com
$390
Product Datasheet

Mouse monoclonal [4B9] antibody to SARS-CoV-2 Spike Protein for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-SARS-CoV-2 Spike Protein Antibody [4B9]
Description
Mouse monoclonal [4B9] antibody to SARS-CoV-2 Spike Protein.
Applications
WB
Dilutions
WB: 1:1,000-1:10,000
Reactivity
SARS-CoV-2
Cross Reactivity
No cross-reactivity observed with SARS-CoV spike protein.
Immunogen
Synthetic peptide corresponding to amino acids 687-704 of SARS-CoV-2 spike protein.
Sequence
VASQSIIAYTMSLGAENS
Host
Mouse
Clonality
Monoclonal
Clone ID
4B9
Isotype
IgG1
Conjugate

Unconjugated

Molecular Weight
141 kDa (but appears as 180 kDa in SDS-PAGE).
Product Form
Lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water. Centrifuge if not clear.
Formulation
Supplied lyophilized from 1X Phosphate Buffered Saline.
Storage
Lyophilized: Store at 2-8°C. Reconstituted: Aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-SARS-CoV-2 Spike Protein Antibody [4B9] (A254398) - Antibodies.comEnlarge Image

Western Blot - Anti-SARS-CoV-2 Spike Protein Antibody [4B9] (A254398)

The GST-Spike (receptor binding domain) recombinant proteins of SARS-CoV-2 (Lane 1) and SARS-CoV (Lane 2) were expressed in E. coli, then bacterial lysate was prepared and resolved in 10% SDS-PAGE. The membrane was probed with Anti-SARS-CoV-2 Spike Protein Antibody [4B9] (A254398) (upper panel), strip washed and subsequently re-probed with Anti-GST Antibody [2E5] (A254385) (lower panel).
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