Figure 1: Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 Antibody (A305449)
Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike S2 Protein, using Anti-SARS-CoV-2 Spike Glycoprotein S2 Antibody (A305449) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Figure 2: Western Blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 Antibody (A305449)
Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-SARS-CoV-2 Spike Glycoprotein S2 Antibody (A305449). This Western blot was performed on the immunoprecipitate using Anti-SARS-CoV-2 Spike Glycoprotein S2 Antibody (A305449) at a dilution of 1:3000.
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