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Anti-Sarcomeric Alpha Actinin Antibody (A14449)

Western Blot - Anti-Sarcomeric Alpha Actinin Antibody (A14449) - Antibodies.com Immunofluorescence - Anti-Sarcomeric Alpha Actinin Antibody (A14449) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Sarcomeric Alpha Actinin for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Sarcomeric Alpha Actinin Antibody
Description
Rabbit polyclonal antibody to Sarcomeric Alpha Actinin.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ACTN2 (NP_001094.1).
Sequence
MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
104 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Actin binding protein, Actinin alpha 2, ACTN 2, ACTN2, ACTN2_HUMAN, Alpha actinin 2, Alpha actinin skeletal muscle, Alpha actinin skeletal muscle isoform 2, Alpha-actinin skeletal muscle isoform 2, Alpha-actinin-2, CMD1AA, F actin cross linking protein, F-actin cross-linking protein
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Sarcomeric Alpha Actinin Antibody (A14449) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Sarcomeric Alpha Actinin Antibody (A14449)
Western blot analysis of extracts of various cell lines, using Anti-Sarcomeric Alpha Actinin Antibody (A14449) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-Sarcomeric Alpha Actinin Antibody (A14449) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Sarcomeric Alpha Actinin Antibody (A14449)
Immunofluorescence analysis of HeLa cells using Anti-Sarcomeric Alpha Actinin Antibody (A14449) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Sarcomeric Alpha Actinin Antibody (A14449)? Please let us know so that we can list the citation on this page.

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