Anti-SAMHD1 Antibody (A15901)

Name: Anti-SAMHD1 Antibody
Brand: Antibodies.com
SKU: A15901
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to SAMHD1 for WB, IHC and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-SAMHD1 Antibody

Description

Rabbit polyclonal antibody to SAMHD1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2).

Sequence

TDAFLKADDYIEITGAGGKKYRISTAIDDMEAYTKLTDNIFLEILYSTDPKLKDAREILKQIEYRNLFKYVGETQPTGQIKIKREDYESLPKEVASAKPKVLLDVKLKAEDFIVDVINMDYGMQEKNPIDHVSFYCKTAPNRAIRITKNQVSQLLPEKFAEQLIRVYCKKVDRKSLYAARQYFVQWCADRNFTKPQDGDVIAPLITPQKKEWNDSTSVQNPTRLREASKSRVQLFKDDPM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

72 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CHBL2, DCIP, Dendritic cell derived IFNG induced protein, Dendritic cell-derived IFNG-induced protein, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, HD domain containing 1, HDDC1, Mg11, Monocyte protein 5, MOP 5, MOP-5, MOP5, OTTHUMP00000030889, SAM domain and HD domain 1, SAM domain and HD domain containing protein 1, SAM domain and HD domain-containing protein 1, SAMH1_HUMAN, SBBI88

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SAMHD1 Antibody (A15901)

Western blot analysis of extracts of various cell lines, using Anti-SAMHD1 Antibody (A15901) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-SAMHD1 Antibody (A15901)

Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using Anti-SAMHD1 Antibody (A15901) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 3: Immunohistochemistry - Anti-SAMHD1 Antibody (A15901)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-SAMHD1 Antibody (A15901) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-SAMHD1 Antibody (A15901)

Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-SAMHD1 Antibody (A15901). This Western blot was performed on the immunoprecipitate using Anti-SAMHD1 Antibody (A15901) at a dilution of 1:1000.

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