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Anti-SAH Antibody [301-3] (A51755)

Titration Curve - Anti-SAH Antibody from Arthus Biosystems (MAH00301) - Antibodies.com Standard Curve - Anti-SAH Antibody from Arthus Biosystems (MAH00301) - Antibodies.com
$1,695

Mouse monoclonal (301-3) antibody to S-Adenosylhomocysteine (HRP) for cELISA.

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$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-SAH Antibody [301-3]
Description
Mouse monoclonal (301-3) antibody to S-Adenosylhomocysteine (HRP).
Specificity
This antibody shows the following reactivities with related compounds: S-Adenosylhomocysteine: 100%, S-Adenosylmethionine: < 3%, Adenosine: < 1%, Homocysteine: < 1%, L-Cysteine: < 1%, Glutahione: < 1%, L-Cystathionine: < 1%, Methythioadenosine (MTA): < 5%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%.
Applications
ELISA">cELISA
Dilutions
cELISA: 1:1,000 - 1:2,500 (depending on the amount of coating).
Immunogen
S-Adenosylhomocysteine conjugated to BSA.
Host
Mouse
Clonality
Monoclonal
Clone ID
301-3
Isotype
IgG3
Conjugate

HRP

Purification
Affinity chromatography
Purity
> 95%
Product Form
Liquid
Formulation
Supplied in 10mM PBS, pH 7.4, with 150mM NaCl, 0.1% Proclin, 50% Glycerol, and 10mg/ml BSA.
Storage
Store at -20°C.
Synonyms
Adenosyl Homocysteine, S (5' Adenosyl) L homocysteine, S Adenosyl L homocysteine, S adenosylhomocysteine
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Titration Curve - Anti-SAH Antibody from Arthus Biosystems (MAH00301) - Antibodies.comEnlarge Image
Figure 1: Anti-SAH Antibody [301-3] (A51755)
Titer of MAH00301. The 0.5 µl/ml of SAH coating standard (Cat # ACT00301 Lot # ACP420210) was coated on micro-titer wells (the top curve). The MAH00301 was serially diluted and added to the wells. After incubation of about 60 minutes, substrates were added and OD450 was measured. The flat curve in the bottom showed the experiment control where no SAH was coated to the plate.
Standard Curve - Anti-SAH Antibody from Arthus Biosystems (MAH00301) - Antibodies.comEnlarge Image
Figure 2: Standard Curve - Anti-SAH Antibody [301-3] (A51755)
The standard curve with MAH00301 in direct competitive ELISA. The 0.5 µg/ml of SAH coating standard (Cat # ACT00301 Lot # ACP420210) was coated on micro-titer wells. Serial dilution of SAH standard (Cat # AST00301) was added along with 1:1,500 diluted MAH00301 and was incubated for about 60 minutes. The substrates were then added and OD450 was measured.
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