Anti-SAH Antibody [301-3] (A51757)

Immunofluorescence (IF) staining of HepG2 and L02 cells double stained with MAF00301 at 20 µg/ml and Anti-SAM Antibody (AF647) (#MAF00201) at 4 µg/ml followed by DAPI staining and photographed under the laser scanning confocal miscroscope Zeiss LSM 78 (x630). (A1-A4) Normal liver cell line L02 cells cultured in RPMI 1640 with 10% FBS for 40h. Different views are as follows: DAPI (A1); AF488 for SAH (2); AF647 for SAM (A3); Overlap of all the three fluorescent signals (A4). (B1-B4) Hepatocellular carcinoma cell line HepG2 cells cultured for 40h (100% confluent). Different views are follows: DAPI (B1); AF488 for SAH (B2); AF647 for SAM (B3); Overlap of all the three fluorescent signals (B4). Both antibodies were used at relatively low concentrations. When cells are 100% confluent, both SAM and SAH are mainly seen around nuclear membrane.

Flow Cytometry of L02 (A1-A4) and HepG2 (B1-B4) cells double stained with MAF00301 at 28 µg/ml and MAF00201 at 4.5 µg/ml. 100% confluent cells (cultured for 48h) were fixed and permeabilised with the intracellular fixation / permeabilisation buffer (A2, B2) or the nuclear fixation / permeabilisation buffer (A4, B4) and then double stained with antibodies indicated above. A1 and B1 are the unstained control for A2 and B2 respectively. A3 and B3 are the unstained control for A4 and B4 respectively. Cells were used for analysis with BD FACS Calibur Flow Cytometer. Both fluorescent signals in A4 and B4 are higher than those of A2 and B2.