Anti-S6K1 Antibody (A13944)

Name: Anti-S6K1 Antibody
Brand: Antibodies.com
SKU: A13944
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to S6K1 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Ribosomal Marker

Specifications

Name

Anti-S6K1 Antibody

Description

Rabbit polyclonal antibody to S6K1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-532 of human P70 S6K (NP_003152.1).

Sequence

KFSPGDFWGRGASASTANPQTPVEYPMETSGIEQMDVTMSGEASAPLPIRQPNSGPYKKQAFPMISKRPEHLRMNL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

70 kDa ribosomal protein S6 kinase 1, KS6B1_HUMAN, p70 alpha, P70 beta 1, p70 ribosomal S6 kinase alpha, p70 ribosomal S6 kinase beta 1, P70 S6 Kinase, p70 S6 kinase alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K, p70 S6K-alpha, p70 S6KA, p70(S6K) alpha, p70(S6K)-alpha, p70-alpha, p70-S6K, p70-S6K 1, P70S6K, P70S6K1, p70S6Kb, PS6K, Ribosomal protein S6 kinase 70kDa polypeptide 1, Ribosomal protein S6 kinase beta 1, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K-beta-1, Serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A, STK14A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-S6K1 Antibody (A13944)

Western blot analysis of extracts of various cell lines, using Anti-S6K1 Antibody (A13944) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-S6K1 Antibody (A13944)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-S6K1 Antibody (A13944) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunofluorescence - Anti-S6K1 Antibody (A13944)

Immunofluorescence analysis of C6 cells using Anti-S6K1 Antibody (A13944) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 4: Immunofluorescence - Anti-S6K1 Antibody (A13944)

Immunofluorescence analysis of NIH-3T3 cells using Anti-S6K1 Antibody (A13944) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).