Western blot analysis of extracts of various cell lines, using Anti-S100 beta Antibody (A17137) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-S100 beta Antibody (A17137) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of U-251 MG cells using Anti-S100 beta Antibody (A17137) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 600µg extracts of mouse brain cells using 3µg of Anti-S100 beta Antibody (A17137). This Western blot was performed on the immunoprecipitate using Anti-S100 beta Antibody (A17137) at a dilution of 1:500.
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