Anti-S100A9 Antibody (A84636)

Name: Anti-S100A9 Antibody
Brand: Antibodies.com
SKU: A84636
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to S100A9 for ELISA, WB, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-S100A9 Antibody

Description

Goat polyclonal antibody to S100A9.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:8,000, WB: 0.5-2µg/ml, IHC: 2.5ug/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human S100A9.

Sequence

C-DTNADKQLSFEEF

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

13-14 kDa

Predicted MW

13.2kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

60B8AG, CAGB, Calgranulin B, Calgranulin-B, Calprotectin L1H subunit, CFAG, CGLB, Cystic fibrosis antigen B, L1AG, Leukocyte L1 complex heavy chain, LIAG, MAC387, MIF, Migration inhibitory factor related protein 14, Migration inhibitory factor-related protein 14, MRP 14, MRP-14, MRP14, Myeloid-related protein 14, NIF, OTTHUMP00000015331, p14, Protein S100-A9, S100 A9, S100 calcium binding protein A9, S100 calcium binding protein A9 calgranulin B, S100 calcium-binding protein A9, S10A9_HUMAN

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-S100A9 Antibody (A84636)

S100A9 expression in Human Bone Marrow (A), Gastrointestinal cancer (B), and negative control HepG2 (C) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 5µg protein was run per lane. Primary antibody was performed with Anti-S100A9 Antibody (A84636) at 1µg/ml (A) and 0.5µg/ml (B) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-S100A9 Antibody (A84636)

S100A9 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-S100A9 Antibody (A84636)

S100A9 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-S100A9 Antibody (A84636)

S100A9 expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-S100A9 Antibody (A84636) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.