Anti-S100A7 Antibody (A84218) (0.1µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human S100A7 with C-terminal MYC tag probed with Anti-S100A7 Antibody (A84218) (0.5µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with Anti-S100A7 Antibody (A84218) (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.