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Anti-S100A4 Antibody [ARC0475] (A305282)

Western Blot - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.com Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.com Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.com Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.com Immunofluorescence - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.com Immunofluorescence - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.com Immunofluorescence - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0475] antibody to S100A4 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-S100A4 Antibody [ARC0475]
Description
Rabbit monoclonal [ARC0475] antibody to S100A4.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2-101 of human S100A4 (P26447).
Sequence
ACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0475
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
12 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
18A2, 42A, calcium Placental protein, Calvasculin, CAPL, Fibroblast specific protein, Fibroblast specific protein 1, Fibroblast specific protein 1 (FSP1), FSP1, Leukemia multidrug resistance associated protein, Malignant transformation suppression 1, Malignant transformation suppression 1 (MTS1), Metastasin, MTS1, OTTHUMP00000015467, OTTHUMP00000015468, P9KA, PEL98, Placental calcium-binding protein, Protein Mts1, Protein S100 A4, Protein S100-A4, S100 calcium binding protein A4, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S10A4_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-S100A4 Antibody [ARC0475] (A305282)
Western blot analysis of extracts of various cell lines, using Anti-S100A4 Antibody [ARC0475] (A305282) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-S100A4 Antibody [ARC0475] (A305282)
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-S100A4 Antibody [ARC0475] (A305282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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