Anti-S100A11 Antibody (A14798)

Name: Anti-S100A11 Antibody
Brand: Antibodies.com
SKU: A14798
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to S100A11 for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-S100A11 Antibody

Description

Rabbit polyclonal antibody to S100A11.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human S100A11 (NP_005611.1).

Sequence

MAKISSPTETERCIESLIAVFQKYAGKDGYNYTLSKTEFLSFMNTELAAFTKNQKDPGVLDRMMKKLDTNSDGQLDFSEFLNLIGGLAMACHDSFLKAVPSQKRT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

12 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Calgizzarin, Epididymis secretory protein Li 43, HEL S 43, Metastatic lymph node gene 70 protein, MLN 70, MLN70, Protein S100 A11, Protein S100-A11, N-terminally processed, Protein S100-C, Protein S100A11, Protein S100C, S100 A11, S100 calcium binding protein A11, S100 calcium-binding protein A11, S100 calcium-binding protein A11 (calgizzarin), S100C, S10AB_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-S100A11 Antibody (A14798)

Western blot analysis of extracts of various cell lines, using Anti-S100A11 Antibody (A14798) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-S100A11 Antibody (A14798)

Western blot analysis of extracts from normal (control) and S100A11 knockout (KO) HeLa cells, using Anti-S100A11 Antibody (A14798) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunofluorescence - Anti-S100A11 Antibody (A14798)

Immunofluorescence analysis of U2OS cells using Anti-S100A11 Antibody (A14798). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-S100A11 Antibody (A14798)

Immunoprecipitation analysis of 200µg extracts of THP-1 cells using 3µg of Anti-S100A11 Antibody (A14798). This Western blot was performed on the immunoprecipitate using Anti-S100A11 Antibody (A14798) at a dilution of 1:1000.

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