Anti-RUNX2 Antibody (A11571)

Name: Anti-RUNX2 Antibody
Brand: Antibodies.com
SKU: A11571
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to RUNX2 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RUNX2 Antibody

Description

Rabbit polyclonal antibody to RUNX2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human RUNX2 (NP_001019801.3).

Sequence

TITVFTNPPQVATYHRAIKVTVDGPREPRRHRQKLDDSKPSLFSDRLSDLGRIPHPSMRVGVPPQNPRPSLNSAPSPFNPQGQSQITDPRQAQSSPPWSYD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Acute myeloid leukemia 3 protein, AML3, CBF-alpha-1, CBFA1, Core-binding factor subunit alpha-1, Oncogene AML-3, OSF-2, OSF2, Osteoblast-specific transcription factor 2, PEA2-alpha A, PEBP2-alpha A, PEBP2A, Polyomavirus enhancer-binding protein 2 alpha A subunit, Runt-related transcription factor 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-RUNX2 Antibody (A11571)

Western blot analysis of extracts of various cell lines, using Anti-RUNX2 Antibody (A11571) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Western Blot - Anti-RUNX2 Antibody (A11571)

Western blot analysis of extracts of Mouse testis, using Anti-RUNX2 Antibody (A11571) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-RUNX2 Antibody (A11571)

Immunohistochemistry analysis of paraffin-embedded mouse salivary gland using Anti-RUNX2 Antibody (A11571) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-RUNX2 Antibody (A11571)

Immunofluorescence analysis of rat spleen cells using Anti-RUNX2 Antibody (A11571) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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