Anti-RRM1 Antibody (A13328)

Name: Anti-RRM1 Antibody
Brand: Antibodies.com
SKU: A13328
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to RRM1 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-RRM1 Antibody

Description

Rabbit polyclonal antibody to RRM1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 593-792 of human RRM1 (NP_001024.1).

Sequence

IRNSLLIAPMPTASTAQILGNNESIEPYTSNIYTRRVLSGEFQIVNPHLLKDLTERGLWHEEMKNQIIACNGSIQSIPEIPDDLKQLYKTVWEISQKTVLKMAAERGAFIDQSQSLNIHIAEPNYGKLTSMHFYGWKQGLKTGMYYLRTRPAANPIQFTLNKEKLKDKEKVSKEEEEKERNTAAMVCSLENRDECLMCGS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

R1, Ribonucleoside diphosphate reductase large subunit, Ribonucleoside diphosphate reductase M1 chain, Ribonucleoside diphosphate reductase subunit M1, Ribonucleoside reductase, large subunit, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleotide reductase large chain, Ribonucleotide reductase large subunit, Ribonucleotide reductase M1, Ribonucleotide reductase M1 polypeptide, Ribonucleotide reductase R1 subunit, Ribonucleotide reductase, M1 subunit, RIR 1, RIR1, RIR1_HUMAN, RR 1, RR1, RRM 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-RRM1 Antibody (A13328)

Western blot analysis of extracts of various cell lines, using Anti-RRM1 Antibody (A13328) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-RRM1 Antibody (A13328)

Western blot analysis of extracts of Rat lung, using Anti-RRM1 Antibody (A13328) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-RRM1 Antibody (A13328)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-RRM1 Antibody (A13328) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-RRM1 Antibody (A13328)

Immunofluorescence analysis of HeLa cells using Anti-RRM1 Antibody (A13328) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).