Western blot analysis of various lysates, using Anti-RPS10 Antibody (A15031) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-RPS10 Antibody (A15031) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-RPS10 Antibody (A15031) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Anti-RPS10 Antibody (A15031) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 1µg of Anti-RPS10 Antibody (A15031). This Western blot was performed on the immunoprecipitate using Anti-RPS10 Antibody (A15031) at a dilution of 1:1000.
