Anti-RPL22 Antibody (A88381)

Name: Anti-RPL22 Antibody
Brand: Antibodies.com
SKU: A88381
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to RPL22 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-RPL22 Antibody

Description

Rabbit polyclonal antibody to RPL22.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1).

Sequence

MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

60S ribosomal protein L22, EAP, EBER associated protein, EBER-associated protein, Epstein Barr encoded RNA associated protein, Epstein Barr virus small RNA associated protein, Epstein-Barr virus small RNA-associated protein, HBP15, HBP15 L22, heparin binding protein 15, Heparin binding protein HBp15, Heparin-binding protein HBp15, ribosomal protein L22, RL22_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-RPL22 Antibody (A88381)

Western blot analysis of extracts of various cell lines, using Anti-RPL22 Antibody (A88381) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-RPL22 Antibody (A88381)

Western blot analysis of extracts from normal (control) and RPL22 knockout (KO) 293T cells, using Anti-RPL22 Antibody (A88381) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-RPL22 Antibody (A88381)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-RPL22 Antibody (A88381) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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