Anti-RPE65 Antibody [ARC1659] (A308904)

Name: Anti-RPE65 Antibody [ARC1659]
Brand: Antibodies.com
SKU: A308904
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1659] antibody to RPE65 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RPE65 Antibody [ARC1659]

Description

Rabbit monoclonal [ARC1659] antibody to RPE65.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human RPE65 (Q16518).

Sequence

TIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1659

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

All-trans-retinyl-palmitate hydrolase, Lutein isomerase, Meso-zeaxanthin isomerase, Retinal pigment epithelium-specific 65 kDa protein, Retinoid isomerohydrolase, Retinol isomerase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-RPE65 Antibody [ARC1659] (A308904)

Western blot analysis of extracts of Rat eye, using Anti-RPE65 Antibody [ARC1659] (A308904) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-RPE65 Antibody [ARC1659] (A308904)

Immunohistochemistry analysis of paraffin-embedded rat retina using Anti-RPE65 Antibody [ARC1659] (A308904) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-RPE65 Antibody [ARC1659] (A308904)

Immunohistochemistry analysis of paraffin-embedded mouse retina using Anti-RPE65 Antibody [ARC1659] (A308904) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-RPE65 Antibody [ARC1659] (A308904)

Immunofluorescence analysis of rat eye using Anti-RPE65 Antibody [ARC1659] (A308904) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).