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Neuroscience
Neurology Process
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Axonal Guidance Proteins
Anti-Robo1 Antibody (A83460)
Overview
Specifications
Images
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$480
Product Datasheet
Goat polyclonal antibody to Robo1 for ELISA, IF and IHC.
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Product Size:
100µg
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$40 to United States
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3-5 Business Days
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Specifications
Name
Anti-Robo1 Antibody
Description
Goat polyclonal antibody to Robo1.
Applications
ELISA
,
IF
,
IHC
Dilutions
ELISA: 1:16,000, IHC: 5-6µg/ml, IF: 10µg/ml
Reactivity
Human
Cross Reactivity
This antibody is not expected to cross react with ROBO2 or ROBO3.
Immunogen
Synthetic peptide corresponding to the internal region of human Robo1.
Sequence
C-GDVDLSNKINEMK
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Deleted in U twenty twenty, DUTT 1, DUTT1, FLJ21882, H Robo 1, H-Robo-1, hRobo 1, Robo 1, ROBO1_HUMAN, Roundabout 1, Roundabout axon guidance receptor homolog 1, Roundabout homolog 1, Roundabout homolog1 precurser, Roundabout1, SAX 3, SAX3
Isotype Controls
Goat IgG (A121671)
Suitable Secondaries
Donkey Anti-Goat IgG H&L Antibody (AP) (A300679)
Donkey Anti-Goat IgG H&L Antibody (Biotin) (A300716)
Donkey Anti-Goat IgG H&L Antibody (FITC) (A300685)
Donkey Anti-Goat IgG H&L Antibody (HRP) (A300730)
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (4)
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Figure 1: Western Blot - Anti-Robo1 Antibody (A83460)
Anti-ROBO1 Antibody (A83460) (1µg/ml) staining of HUVEC lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Figure 2: Immunohistochemistry - Anti-Robo1 Antibody (A83460)
Anti-ROBO1 Antibody (A83460) (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Figure 3: Immunofluorescence - Anti-Robo1 Antibody (A83460)
Anti-ROBO1 Antibody (A83460) - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing Golgi and membrane staining.
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Figure 4: Flow Cytometry - Anti-Robo1 Antibody (A83460)
Anti-ROBO1 Antibody (A83460) - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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