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Anti-RNF31 / HOIP Antibody (A87886)

Western Blot - Anti-RNF31 / HOIP Antibody (A87886) - Antibodies.com Immunofluorescence - Anti-RNF31 / HOIP Antibody (A87886) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to RNF31 / HOIP for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-RNF31 / HOIP Antibody
Description
Rabbit polyclonal antibody to RNF31 / HOIP.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human RNF31 (NP_060469.4).
Sequence
AAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
120 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-RNF31 / HOIP Antibody (A87886) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-RNF31 / HOIP Antibody (A87886)
Western blot analysis of extracts of various cell lines, using Anti-RNF31 / HOIP Antibody (A87886) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-RNF31 / HOIP Antibody (A87886) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-RNF31 / HOIP Antibody (A87886)
Immunofluorescence analysis of human breast using Anti-RNF31 / HOIP Antibody (A87886) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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