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Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser7) Antibody (A308770)

Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser7) Antibody (A308770) - Antibodies.com
$470
Product Datasheet

Rabbit polyclonal antibody to RNA polymerase II CTD repeat YSPTSPS (phospho Ser7) for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser7) Antibody
Description
Rabbit polyclonal antibody to RNA polymerase II CTD repeat YSPTSPS (phospho Ser7).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse
Immunogen
A phospho specific peptide corresponding to residues surrounding S7 of human POLR2A CTD repeat YSPTSPS.
Sequence
SPSYS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
270 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A, Polymerase (RNA) II (DNA directed) polypeptide A 220kDa, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB1_HUMAN, RPBh1, RpIILS, RPO2, RPOL2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser7) Antibody (A308770) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser7) Antibody (A308770)
Western blot analysis of extracts of MCF7 cells, using Anti-RNA polymerase II CTD repeat YSPTSPS (phospho Ser7) Antibody (A308770) at 1:1,000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37°C for 24 hours. MCF7 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
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