Anti-RNA Polymerase II CTD repeat YSPTSPS Antibody [CTD4H8] - BSA and Azide free (A252834)

Mouse monoclonal [CTD4H8] antibody to RNA Polymerase II CTD repeat YSPTSPS for Flow Cytometry, IF, WB and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-RNA Polymerase II CTD repeat YSPTSPS Antibody [CTD4H8] - BSA and Azide free
Description
Mouse monoclonal [CTD4H8] antibody to RNA Polymerase II CTD repeat YSPTSPS.
Specificity
RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
Applications
Flow Cytometry, IF, WB, IHC-P
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse, Rat
Immunogen
Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho Ser5.
Host
Mouse
Clonality
Monoclonal
Clone ID
CTD4H8
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
192-253 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-RNA Polymerase II CTD repeat YSPTSPS Antibody [CTD4H8] (A249654).
Synonyms
DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A, Polymerase (RNA) II (DNA directed) polypeptide A 220kDa, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RPB1, RPB1_HUMAN, RPBh1, RpIILS, RPO2, RPOL2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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