Synthetic peptide within amino acids 1650 and the C-terminus of human RICTOR.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
AVO3 homolog, hAVO3, KIAA1999, Rapamycin-insensitive companion of mTOR
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-RICTOR Antibody (A295540) was used for IP at 3 µg per reaction. RICTOR was also immunoprecipitated by a previous lot of this antibody and another rabbit anti-RICTOR antibody. For blotting immunoprecipitated RICTOR, Anti-RICTOR Antibody (A295540) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Anti-RICTOR Antibody (A295540) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Anti-RICTOR Antibody (A295540) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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