Anti-Rhodopsin Antibody [A531] (A85374)

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat retina section with Anti-Rhodopsin Antibody [A531] (A85374) at a dilution of 1:2,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). Anti-Rhodopsin Antibody [A531] (A85374) strongly labels the rod cells within the photoreceptor layer of the retina. Note: Note: this antibody has not been tested in NBF fixed material.

Pig retinal section stained with Anti-Rhodopsin Antibody (green), Anti-NF-M Antibody (A85323 | red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).

Immunofluorescent analysis of mouse retina section stained with Anti-Rhodopsin Antibody [A531] (A85374), at a dilution of 1:2,000, in green, and co-stained with Anti-GAP43 Antibody (A85394), at a dilution of 1:1,000 in red. The nuclear DNA is visualised in blue using Hoechst staining. The Anti-Rhodopsin Antibody [A531] (A85374) reveals rhodopsin protein in rod cell membranes located in outer segments of photoreceptors layer (OS) of retina. The Anti-GAP43 Antibody (A85394) stains axons of neuronal cells in the inner plexiform layer (IPL), where it was present in three distinct bands.

Western blot analysis of retinal lysates from different species using Anti-Rhodopsin Antibody [A531] (A85374), at a dilution of 1:5,000, in green. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] rat [Lane 3] mouse and [Lane 4] cow retina lysates. The strong band at 35 kDa corresponds to rhodopsin protein. Bands around 70 kDa and 140 kDa result from the known tendency of rhodopsin to aggregate on SDS-PAGE gels.