This antibody recognises native and recombinant forms of Rhodopsin. It binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH). There is no known reactivity to other proteins.
Applications
WB, IHC, ICC/IF, IP, ELISA
Dilutions
WB: 1:1,000, IHC: 1:100
Reactivity
Vertebrate
Immunogen
Bovine Rhodopsin.
Host
Mouse
Clonality
Monoclonal
Clone ID
1D4
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
1 mg/ml
Molecular Weight
~40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Western Blot - Anti-Rhodopsin Antibody [1D4] (A305239)
Western blot analysis of human Cell lysates showing detection of Rhodopsin protein using Anti-Rhodopsin Antibody [1D4] (A305239) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-Rhodopsin Antibody [1D4] (A305239) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Dull epidermal staining.
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