This antibody recognises the sequence TETSQVAPA corresponding to aa 340 to 348 of bovine rhodopsin, a pigment of the retina responsible for the formation of photoreceptor cells and the first events in the perception of light. Rhodopsin consists of the protein opsin and a reversibly bound cofactor, retinal, a photoreactive chromophore. Upon light absorption the 11-cis-retinal is isomerised to all-trans retinal, leading to a change in the shape of rhodopsin. The change activates the associated G protein and triggers a second messenger cascade, eventually resulting in the transmission of a nerve impulse to the brain. The trans-retinal is released and converted back into 11-cis-retinal for re-entry into the cycle.Defects in the gene cause various retinopathies such as retinitis pigmentosa and X-linked congenital stationary night blindness.
Applications
WB, IHC-P, IP, IF
Dilutions
IHC-P: 1:100, WB: 1:1,000
Reactivity
Bovine, Mammals, Amphibia
Immunogen
Bovine rhodopsin.
Host
Mouse
Clonality
Monoclonal
Clone ID
1D4
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in 10mM HEPES, pH 7.5, with 150mM NaCl, 0.01% BSA, 50% Glycerol, and 0.05% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rhodopsin antibody, clone 1D4 recognizes the sequence TETSQVAPA corresponding to aa 340 to 348 of bovine rhodopsin, a pigment of the retina responsible for the formation of photoreceptor cells and the first events in the perception of light. Rhodopsin consists of the protein opsin and a reversibly bound cofactor, retinal, a photoreactive chromophore. Upon light absorption the 11-cis-retinal is isomerised to all-trans retinal, leading to a change in the shape of rhodopsin. The change activates the associated G protein and triggers a second messenger cascade, eventually resulting in the transmission of a nerve impulse to the brain. The trans-retinal is released and converted back into 11-cis-retinal for re-entry into the cycle.Defects in the gene cause various retinopathies such as retinitis pigmentosa and X-linked congenital stationary night blindness.