Anti-RhoA Antibody (A91750)

Name: Anti-RhoA Antibody
Brand: Antibodies.com
SKU: A91750
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to RhoA for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-RhoA Antibody

Description

Rabbit polyclonal antibody to RhoA.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 94-193 of human RhoA (NP_001655.1).

Sequence

NIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Aplysia ras related homolog 12, ARH12, ARHA, H 12, H12, Oncogene RHO H12, Ras homolog family member A, Ras homolog gene family member A, Rho A, Rho cDNA clone 12, RHO H12, RHO12, RHOA_HUMAN, RHOH12, Small GTP binding protein Rho A, Transforming protein Rho A, Transforming protein RhoA

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-RhoA Antibody (A91750)

Western blot analysis of extracts of various cell lines, using Anti-RhoA Antibody (A91750) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-RhoA Antibody (A91750)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-RhoA Antibody (A91750) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Western Blot - Anti-RhoA Antibody (A91750)

Immunoprecipitation analysis of 200µg extracts of NIH/3T3 cells using 3µg of Anti-RhoA Antibody (A91750). This Western blot was performed on the immunoprecipitate using Anti-RhoA Antibody (A91750) at a dilution of 1:500.

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